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Journal of Sistan and Baluchistan Studies

Journal of Sistan and Baluchistan Studies

GENDER-BASED DIETARY DIFFERENCES AT MERSIN CHAL CEMETERY DURING THE SECOND HALF OF THE FIRST MILLENNIUM

Document Type : Original Research Article

Authors
1 Postdoctoral Researcher in Archaeology, Tarbiat Modares University, Tehran, Iran.
2 Professor of Archaeology, Department of Archaeology, Tarbiat Modares University, Tehran, Iran.
3 Associate Professor of Archaeology, Iranian Center for Archaeological Research, Tehran, Iran.
10.22034/jsbs.2026.543327.1105
Abstract
The Mersin Chal cemetery is situated in the village of Telajim, within Mehdi Shahr city in Semnan Province. During the third season of archaeological excavations at the Mersin Chal cemetery in 2021, a total of 49 burials were identified, dating back to the second half of the first millennium BC. One of the main focuses in bioarchaeological research involves examining how physiological differences between sexes influence dietary patterns, often analyzed through the stable isotope δ15N. This research aimed to investigate the biological differences in the diets of individuals from the Mersin Chal cemetery through bioarchaeological analysis. Aligned with this goal, a question emerged regarding whether the gender differences between males and females result in variations in their nutritional mechanisms. This research addresses the question by analyzing human skeletal remains from 12 of the 49 excavated graves in the Mersin Chal cemetery. This research employs δ15N stable isotope analysis on dental collagen samples, combined with SPSS statistical methods, to interpret the findings. The analysis of δ15N stable isotope ratios from adult male and female skeletons in the Mersin Chal cemetery reveals that males exhibit slightly higher δ15N values compared to females. This suggests greater variation in the amount of protein consumed by males. This difference likely reflects greater protein intake in males, consistent with their higher proportional protein requirements relative to body mass.
Keywords

چکیده

گورستان مِرسین چَل در روستای تلِاجیم، در محدوده شهر مهدی‌شهر استان سمنان واقع شده است. در فصل سوم کاوش‌های باستان‌شناسی در گورستان مِرسین چَل در سال ۱۴۰۰، مجموعاً ۴۹ تدفین شناسایی شد که مربوط به نیمه دوم هزاره اول پیش از میلاد هستند. یکی از تمرکزهای اصلی در پژوهش‌های باستان‌زیست‌شناسی، بررسی چگونگی تأثیر تفاوت‌های فیزیولوژیکی بین جنسیت‌ها بر الگوهای غذایی است که اغلب از طریق ایزوتوپ پایدار δ15N تحلیل می‌شود. این پژوهش با هدف بررسی تفاوت‌های زیستی در رژیم غذایی افراد گورستان مِرسین چَل از طریق تجزیه و تحلیل باستان‌زیست‌شناسی انجام شد. در راستای این هدف، این سوال مطرح شد که آیا تفاوت‌های جنسیتی بین مردان و زنان منجر به تغییراتی در مکانیسم‌های تغذیه‌ای آن‌ها می‌شود؟ این پژوهش با تجزیه و تحلیل بقایای اسکلت انسانی ۱۲ قبر از ۴۹ قبر کاوش شده در گورستان مِرسین چَل به این سوال پاسخ می‌دهد. این تحقیق از تحلیل ایزوتوپ پایدار δ15N بر روی نمونه‌های کلاژن دندان، همراه با روش‌های آماری SPSS، برای تفسیر یافته‌ها استفاده می‌کند. تجزیه و تحلیل نسبت‌های ایزوتوپ پایدار δ15N از اسکلت‌های مردان و زنان بالغ در گورستان مِرسین چَل نشان می‌دهد که مردان مقادیر کمی بالاتر δ15N نسبت به زنان دارند. این نشان‌دهنده تنوع بیشتر در میزان پروتئین مصرفی توسط مردان است. این تفاوت احتمالاً نشان‌دهنده مصرف پروتئین بیشتر در مردان است که با نیازهای پروتئینی متناسب با توده بدنی بالاتر آن‌ها مطابقت دارد.

کلیدواژه: تحلیل ایزوتوپ پایدار، کلاژن دندان انسان، رژیم غذایی، جنسیت، δ15N.

Agarwal, S.C. & Glencross, B.A. (2011). Social Bioarchaeology, Wiley-Blackwell Publishing Ltd.
Ambrose, S.H. (1990). “Preparation and Characterization of Bone and Tooth Collagen for Isotopic Analysis”. Journal of Archaeological Science 17: 431-451. https://doi.org/10.1016/0305-4403(90)90007-R
Ambrose, S.H., & Lynette, N. (1993). Experimental Evidence for the Relationship of the Carbon Isotope Ratios of Whole Diet and Dietary Protein to Those of Bone Collagen and Carbonate, B. Lambert et al. (eds.), Prehistoric Human Bone, Springer-Verlag Berlin Heidelberg.
Bakhtiari, S., Mousavi, S.M., Nemati, M.R., & Soltysiak, A.” Human Diet and Subsistence at Mersin Chal, an Achaemenid/Seleucid/Parthian Site in Northern Iran”. Journal of Archaeological Science: Reports (under review). http://dx.doi.org/10.2139/ssrn.4930803
Basu, S., Shailesh, A., Sanyal, P., Mahato, P., Kumar, S., & Sarkar, A. (2015). “Carbon Isotopic Ratios of Modern C3–C4 Plants from the Gangetic Plain, India and Its Implications to Paleovegetational Reconstruction”. Palaeogeography, Palaeoclimatology, Palaeoecology 440: 22–32. https://doi.org/10.1016/j.palaeo.2015.08.012
Budd, Ch., Karul, N., Alpaslan-Roodenberg, S., Galik, A., Schulting, R., & Lillie, M. (2017). “Diet Uniformity at an Early Farming Community in Northwest Anatolia (Turkey): Carbon and Nitrogen Isotope Studies of Bone Collagen at Aktopraklık”. Archaeological and Anthropological Sciences: 1-13. https://doi.org/10.1007/s12520-017-0523-4
Bogaard, A., & Outram, A.K. (2013). “Palaeodiet and Beyond: Stable Isotopes in Bioarchaeology”. World Archaeology 45(3): 333-337. 10.1080/00438243.2013.829272
Buikstra, J.E., & Ubelaker, D.H. (1994). Standards for Data Collection from Human Skeletal Remains. Proceedings of a Seminar at the Field Museum of Natural History, Arkansas Archaeological Survey Research Series, No.44.
Cooper, C.G. Lupo, K.D. Zena, A.G. Schmitt, D.N. & M.P. Richards. (2019). “Stable Isotope Ratio Analysis (C, N, S) of Hair from Modern Humans in Ethiopia Shows Clear Differences Related to Subsistence Regimes”. Archaeological and Anthropological Sciences 11: 3213-3223. https://doi.org/10.1007/s12520-018-0740-5
DeNiro, M.J. (1985). “Post-Mortem Preservation and Alteration of in Vivo Bone Collagen Isotope Ratios about Palaeodietary Reconstruction”. Nature 317: 806–809.
Fetner, R. (2015). The Impact of Climate Change on Subsistence Strategies in Northern Mesopotamia: The Stable Isotope Analysis and Dental Microwear Analysis of Human Remains from Bakr Awa (Iraqi Kurdistan), Ph.D. Dissertation, University of Warsaw.
Hager, L. (1996). “Sex Differences in the Sciatic Notch of Great Apes and Modern Humans”. American Journal of Physical Anthropology 99(2): 287-300. DOI: 10.1002/(SICI)1096-8644(199602)99:2<287: AID-AJPA6>3.0.CO;2-W
Işcan, M.Y., Loth, S.R., & Wright, R.K. (1984). “Metamorphosis at the Sternal Rib End: A New Method to Estimate Age at Death in White Males”. American Journal of Physical Anthropology 65: 147-156. DOI: 10.1002/ajpa.1330650206
JiménezBrobeil, S.A., Charisi, D., Laffranchi, Z., Maroto Benavides, R.M., Delgado Huertas, A., & Milella, M. (2021). “Sex Differences in Diet and Life Conditions in a Rural Medieval Islamic Population from Spain (La Torrecilla, Granada): An Isotopic and Osteological Approach to Gender Differentiation in AlAndalus”. American Journal of Physical Anthropology 175(4): 794-815. DOI: 10.1002/ajpa.24277
Katzenberg, M.A. (2008). Stable Isotope Analysis: A Tool for Studying Past Diet, Demography, and Life History. In Biological Anthropology of the Human Skeleton, M.A. Katzenberg & S.R. Saunders (eds.), Hoboken, NJ: John Wiley & Sons, Inc., 413 – 442.
Kelly, J.F. (2000). “Stable Isotopes of Carbon and Nitrogen in the Study of Avian and Mammalian Trophic Ecology”. Canadian Journal of Zoology 78: 1–27. 10.1139/cjz-78-1-1
Lewis, J., Pike, A.W.G., Coath, C.D., & Evershed, R.P. (2017). “Strontium Concentration, Radiogenic (87Sr/86Sr) and Stable (δ88Sr) Strontium Isotope Systematics in a Controlled Feeding Study”. Star: Science and Technology of Archaeological Research 3(1): 45–57. https://doi.org/10.1080/20548923.2017.1303124
Lovejoy, C.O., Meindle, R.S., Pryzbeck, T.R., & Mensforth, R.P. (1985). “Chronological Metamorphosis of the Auricular Surface of the Ilium: A New Method of Determining Adult Age at Death”. American Journal of Physical Anthropology 68, 15-28. https://doi.org/10.1002/ajpa.1330680103
Makarewicz, Ch.A., & Sealy, J. (2015). “Dietary Reconstruction, Mobility, and the Analysis of Ancient Skeletal Tissues: Expanding the Prospects of Stable Isotope Research in Archaeology”. Journal of Archaeological Science 56: 146-158. https://doi.org/10.1016/j.jas.2015.02.035
Malekzadeh, M., Naseri, R., Boroomandara, S., Cesaretti, A., & Dan, R. (2023). “Preliminary Report of the First Season of Excavation at the Achaemenid Period (Iron Age IV) Cemetery in Mersin, Semnan Province, Iran”. IRAN Journal of British Institute of Persian Studies: 1-15. https://doi.org/10.1080/05786967.2023.2170819
Mays, S. (1998). The Archaeology of Human Bones. London: Routledge.
Meindl, R. S. & Lovejoy, C.O. (1985). “Ectocranial Suture Closure: A Revised Method for the Determination of Skeletal Age at Death Based on the Lateral-Anterior Sutures”. American Journal of Physical Anthropology 68(1): 57–66. 10.1002/ajpa.1330680106
Mortezaei, M. & Maleki, A. (2010). The Archaeological Survey of Finesk Dam Basin. Tehran: Unpublished report prepared for Iranian Centre for Archaeological Research of Research Institute of Cultural Heritage and Tourism (in Persian).
Nemati, M. (2021). The Third Season of Archaeological Excavation of Mersin Chal Cemetery (Finesk Dam Basin). Tehran: Unpublished report prepared for ICHHTO (in Persian).
Oliveira, R.N. Silva, S.F.S.M. Kawano, A., & Antunes, J.L.F. (2006). “Estimating Age by Tooth Wear of Prehistoric Human Remains in Brazilian Archaeological Sites”. International Journal of Osteoarchaeology 16: 407–414. https://doi.org/10.1002/oa.840
Price, T.D. (2015).An Introduction to the Isotopic Studies of Ancient Human Remains”.  Journal of the North Atlantic 7:71-87. 10.3721/037.002.sp708
Richards, M.P. (2019). “Isotope Analysis for Diet Studies”. Archaeological Science: 125-144. 10.1017/9781139013826.006
Rojas-Zambrano, J.G., Rojas-Zambrano, A., & Rojas-Zambrano, A.F. (2025). “Impact of Testosterone on Male Health: A Systematic Review”. Cureus 17(4):1-7. 10.7759/cureus.82917
Schoeninger, M.J. & K. Moore. (1992). “Bone Stable Isotope Studies in Archaeology”. Journal of World Prehistory 6(2): 247-296. https://doi.org/10.1007/BF00975551
Sheikhshoaee, F., & Niknami, K. (2016). “Stable Isotope Analysis to Determining Gender Differences in Ancient Dietary Systems”. Journal of Archaeological Studies 8(1): 77-89 (in Persian). https://doi.org/10.22059/jarcs.2016.59496
Sołtysiak, A. (2020). “Human enamel 87Sr/86Sr evidence of migration and land use patterns at Tell Brak, a Late Chalcolithic urban centre in NE Syria”. Archaeological and Anthropological Sciences 12 (143): 1-7. https://doi.org/10.1007/s12520-020-01104-3
Sołtysiak, A., & Fernandes, R. (2021). “Much Ado about Nothing: Assessing the Impact of the 4.2 kya Event on Human Subsistence Patterns in Northern Mesopotamia Using Stable Isotope Analysis”. Antiquity 95 (383):1145-1160. https://doi.org/10.15184/aqy.2021.117
Sołtysiak, A., & Schutkowski, H. (2018). “Stable Isotopic Evidence for Land Use Patterns in the Middle Euphrates Valley, Syria”. American Journal of Physical Anthropology 166(4): 861–874. https://doi.org/10.1002/ajpa.23480
Somerville, A.D., Goldstein, P.S., Baitzel, S.I., Bruwelheide, K.L., Dahlstedt, A.C., Yzurdiaga, L., Raubenheimer, S., Knudson, K.J., & Schoeninger, M.J. (2015). “Diet and Gender in the Tiwanaku Colonies: Stable Isotope Analysis of Human Bone Collagen and Apatite from Moquegua, Peru”. American Journal of Physical Anthropology 158(3): 408-422. doi: 10.1002/ajpa.22795
Tague, R.G. (1995). “Variation in Pelvic Size Between Males and Females in Nonhuman Anthropoids”. American Journal of Physical Anthropology 97 (3): 213- 233. 10.1002/APA.1330970302
Todd, T.W. (1920). “Age Changes in The Pubic Bone. I. The Male white pubis”. American Journal of Physical Anthropology 3 (3): 285-334.   https://doi.org/10.1002/ajpa.1330030301
Van Klinken, G.J. (1999). Bone Collagen Quality Indicators for Palaeodietary and Radiocarbon Measurements. Journal of Archaeological Science 26: 687–695. https://doi.org/10.1006/jasc.1998.0385
White, T.D., & P.A. Folkens. (2005). The Human Bone Manual. Amsterdam: Elsevier Press.
Volume 6, Issue 1
June 2026
Pages 93-100

  • Receive Date 25 August 2025
  • Revise Date 28 October 2025
  • Accept Date 02 January 2026
  • Publish Date 01 June 2026